Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Embryo
Cell type
Embryonic erythroblasts
NA
NA

Attributes by original data submitter

Sample

source_name
Erythrobl
biomaterial_provider
Mitch Weiss lab
lab
PSU-m
lab description
Hardison - Penn State University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
Erythrobl
cell organism
mouse
cell description
Erythroblast, ter119+ cells from liver
cell sex
B
antibody
H3K36me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K36me3
antibody targetdescription
Histone H3 (tri-methyl K36). Marks regions of RNAPII elongation, including coding and non-coding transcripts.
antibody vendorname
Abcam
antibody vendorid
ab9050
age
E14.5
age description
Embryonic day 14.5
control
None
control description
No special treatment or protocol applies
controlid
wgEncodeEM002350
replicate
1
strain
CD-1
strain description
Multipurpose mouse used for safety testing, aging studies, surgical models and pseudopregnancy.

Sequenced DNA Library

library_name
GSM946543: PSU_ChipSeq_Erythrobl_H3K36me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
194377750
Reads aligned (%)
97.2
Duplicates removed (%)
21.2
Number of peaks
60526 (qval < 1E-05)

mm9

Number of total reads
194377750
Reads aligned (%)
97.0
Duplicates removed (%)
21.2
Number of peaks
60595 (qval < 1E-05)

Base call quality data from DBCLS SRA