Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Embryo
Cell type
Embryonic megakaryocytes
NA
NA

Attributes by original data submitter

Sample

source_name
Megakaryo
biomaterial_provider
Mitch Weiss lab
lab
PSU-m
lab description
Hardison - Penn State University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
Megakaryo
cell organism
mouse
cell description
Megakaryocyte
cell sex
B
antibody
H3K4me3
antibody antibodydescription
rabbit polyclonal. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri methyl K4). Marks promoters that are active or poised to be activated.
antibody vendorname
Millipore
antibody vendorid
07-473
age
E14.5
age description
Embryonic day 14.5
control
Input
control description
Control signal which may be subtracted from experimental raw signal before peaks are called.
controlid
wgEncodeEM002352
replicate
1
strain
CD-1
strain description
Multipurpose mouse used for safety testing, aging studies, surgical models and pseudopregnancy.

Sequenced DNA Library

library_name
GSM946527: PSU_ChipSeq_Megakaryo_H3K4me3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
237345499
Reads aligned (%)
93.4
Duplicates removed (%)
39.6
Number of peaks
73410 (qval < 1E-05)

mm9

Number of total reads
237345499
Reads aligned (%)
93.2
Duplicates removed (%)
39.6
Number of peaks
73506 (qval < 1E-05)

Base call quality data from DBCLS SRA