Antigen

Antigen Class

TFs and others

Antigen

EZH2

Cell type

Cell type Class

Lung

Cell type

NCI-H1299

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma Non-Small Cell

Sample

source_name

lymph node-derived

tissue

lymph node-derived

cell line

H1299

cell type

non-small cell lung carcinoma

chip antibody

anti-EZH2

Sequenced DNA Library

library_name

GSM6164860

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The DNA samples were prepared using the SimpleChIP® Plus Enzymatic Chromatin IP Kit (9005, Cell Signaling Technology). The Illumina standard library construction protocol was used including end repair, A-tailing, adapter ligation and PCR with indexed primers. The paired end sequencing of ChIP-seq was run on the NextSeq 500 system.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

30888709

Reads aligned (%)

95.0

Duplicates removed (%)

2.4

Number of peaks

810 (qval < 1E-05)

hg19

Number of total reads

30888709

Reads aligned (%)

94.3

Duplicates removed (%)

3.0

Number of peaks

567 (qval < 1E-05)