Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Digestive tract
Cell type
KYSE-510
Primary Tissue
Esophagus
Tissue Diagnosis
Carcinoma Squamous Cell

Attributes by original data submitter

Sample

source_name
KYSE510
cell line
KYSE510
cell type
Esophageal squamous cell carcinoma (ESCC)
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. DNA fragments were end-pair repaired foloowed by adapter ligation. Later these fragments were PCR-amplified and size selected (100-300 bp).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
68947224
Reads aligned (%)
93.7
Duplicates removed (%)
3.1
Number of peaks
1952 (qval < 1E-05)

hg19

Number of total reads
68947224
Reads aligned (%)
92.8
Duplicates removed (%)
5.4
Number of peaks
1581 (qval < 1E-05)

Base call quality data from DBCLS SRA