Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

TE7

Primary Tissue

Esophagus

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

TE7

cell line

TE7

cell type

Esophageal squamous cell carcinoma (ESCC)

chip antibody

H3K27Ac

chip antibody vendor

AbCam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. DNA fragments were end-pair repaired foloowed by adapter ligation. Later these fragments were PCR-amplified and size selected (100-300 bp).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

64273075

Reads aligned (%)

98.8

Duplicates removed (%)

3.7

Number of peaks

12409 (qval < 1E-05)

hg19

Number of total reads

64273075

Reads aligned (%)

98.0

Duplicates removed (%)

5.5

Number of peaks

11958 (qval < 1E-05)