Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SRPK2

Cell type

Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease

Attributes by original data submitter

Sample

source_name
SRPK2, MCF10A
cell line
MCF10A cells
chip antibody
anti-SRPK2 (Bethyl Labs, catalog# A302-467A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fast-ChIP protocol as mentioned in DOI: 10.1038/nsmb.2123, 10.1093/nar/gks1472 The samples were sequencing by the Core Genomic Faciity - EMBL using Illumina standard protocols. ChIP-seq libraries were sequenced as single-end 51-bp sequence tags using the standard Illumina pipeline

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
13386416
Reads aligned (%)
38.2
Duplicates removed (%)
55.7
Number of peaks
271 (qval < 1E-05)

hg38

Number of total reads
13386416
Reads aligned (%)
40.1
Duplicates removed (%)
54.1
Number of peaks
189 (qval < 1E-05)

Base call quality data from DBCLS SRA