embryoid bodies, UTX depleted, no RA, H3K27me3 ChIP
cell line
v6.5
cell type
embryonic stem cells (ESCs)
genotype/variation
UTX knockout (UTX -/Y)
treatment
Normal
chip antibody
Anti-H3K27me3 (Millipore: 07-449, lot# 2382150)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Briefly, cells were grown to log phase and fixed with 1% formaldehyde for 10 min at room temperature. The fixed cells were sonicated directly. Exactly 10% of the chromatin was used for the extraction of input DNA. For ChIP, 4 μg of antibodies, including anti-H3K4me3 or anti-H3K27me3 antibodies, was bound to protein A or protein G magnetic beads depending on the antibody origin. The ChIP-Seq libraries were constructed using the TruSeq DNA sample prep kit v2 (Illumina) according to the manufacturer's protocol.