Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

source_name

epidermal keratinocyte

cell type

Normal Human Epidermal Keratinocytes (NHEK)

treatment

scramble

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 10 minutes, then lysed and sonicated prior to pull down with antibodies Libraries were created using the Illumina Tru-Seq adapters with NEBNext reagents

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

26508620

Reads aligned (%)

97.5

Duplicates removed (%)

21.1

Number of peaks

25523 (qval < 1E-05)

hg19

Number of total reads

26508620

Reads aligned (%)

97.4

Duplicates removed (%)

21.3

Number of peaks

25372 (qval < 1E-05)