GSM6132235: HCT116 Spt5degron plusTMP pol IIChIP 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SUPT5H
Cell type
Cell type Class
Digestive tract
Cell type
HCT 116
Primary Tissue
Colon
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
HCT116 Spt5-HA-dhfr
cell line
HCT116
growth conditions
McCoy's 10% FCS, +hygromycin, +G418+TMP
antibody
Rabbit anti pan CTD (Schroeder et al Genes Dev. 14:2435, 2000)
Sequenced DNA Library
library_name
GSM6132235
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was sonicated with a Covaris ME220 ultrasonicator 1-2 mg of cross-linked extract was immunoprecipitated, and prepared for illumina library construction by repair with Klenow, T4 DNA polymerase and T4 polynucleotide kinase, A-tailing with Klenow exo-, ligation to branched adaptors, SPRI bead purification of ~200bp fragments, and amplification (15-18 cycles) with Illumina Tru-seq primers using Phusion DNA polymerase.