GSM6124617: PAF1 TOPmentation in RS4;11 cells; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
PAF1
Cell type
Cell type Class
Blood
Cell type
RS4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
Leukemia cell line (RS4;11)
treatment
N/A
cell type
Pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
chip antibody
Bethyl A300-172A
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM6124617
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were washed with PBS and fixed in either 1% FA for 10 minutes (histones and HT-CM) or 2mM DSG for 30 minutes and 1% FA for 30 minutes (TFs). Cells were sonicated to give fragments of 100-300bp followed by IP. IPed chromatin was tagmented by incubating in 29 µl Tagmentation Buffer (10 mM Tris-HCl pH 8.0, 5 mM MgCl2, 10% dimethylformamide) and adding 1 µl of transposase (Illumina) at 37 ˚C for 10 min. Tagmented DNA was amplified and indexed with NEBNext Ultra II Q5 Master Mix (NEB).