Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pancreas
Cell type
PANC-1
Tissue
Pancreas/Duct
Disease
Epithelioid Carcinoma

Attributes by original data submitter

Sample

source_name
PANC-1
biomaterial_provider
ATCC
lab
USC
lab description
Farnham - University of Southern California
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
PANC-1
cell organism
human
cell description
pancreatic carcinoma, (PMID: 1140870) PANC-1 was established from a pancreatic carcinoma, which was extracted via pancreatico-duodenectomy specimen from a 56-year-old Caucasian individual. Malignancy of this cell line was verified via in vitro and in vivo assays.
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
H3K4me3B
antibody antibodydescription
Rabbit monoclonal antibody. Antibody Target: H3K4me3
antibody targetdescription
Histone H3 (tri-methyl K4) marks promoters in chromatin regions
antibody vendorname
Cell Signaling
antibody vendorid
9751S
control
UCDavis
control description
Input library was prepared at UC Davis.
control
UCDavis
control description
Input library was prepared at UC Davis.
controlid
wgEncodeEH002070
replicate
1

Sequenced DNA Library

library_name
GSM945856: USC_ChipSeq_PANC-1_H3K4me3B_UCDavis
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
53620150
Reads aligned (%)
97.7
Duplicates removed (%)
34.5
Number of peaks
29749 (qval < 1E-05)

hg19

Number of total reads
53620150
Reads aligned (%)
97.5
Duplicates removed (%)
34.9
Number of peaks
29597 (qval < 1E-05)

Base call quality data from DBCLS SRA