Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MECOM

Cell type

Cell type Class
Blood
Cell type
Acute myeloid leukemia
NA
NA

Attributes by original data submitter

Sample

source_name
acute myeloid leukemia cell line
rearrangement of chromosome 3q
Yes, t(3;3)(q21;q26)
sf3b1 mutations
Yes, K700E
chip antibody
Anti-EVI1 (Cell Signaling, 2593)

Sequenced DNA Library

library_name
GSM6106333
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclear lysates were sonicated and pulled down with antibody. ChIP samples were processed according to the Illumina TruSeq ChIP Sample Preparation Protocol (Illumina) or Diagenode Library V3 preparation protocol (Diagenode) and either sequenced single-end (1 × 50 bp) on the HiSeq 2500 platform (Illumina) or paired-end (2 × 100 bp) on the Novaseq 6000 platform (Illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
193075142
Reads aligned (%)
99.7
Duplicates removed (%)
79.3
Number of peaks
19294 (qval < 1E-05)

hg19

Number of total reads
193075142
Reads aligned (%)
99.7
Duplicates removed (%)
79.6
Number of peaks
19025 (qval < 1E-05)

Base call quality data from DBCLS SRA