Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

preB_p20

cell type

preB derived p20 iPS

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed as described previously (van Oevelen et al., 2008) using an antibody against H3K27ac (ab4729, Abcam) and CTCF (Millipore, 07-729). DNA libraries were prepared using Illumina's reagents and instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

28328456

Reads aligned (%)

93.5

Duplicates removed (%)

11.0

Number of peaks

19943 (qval < 1E-05)

mm9

Number of total reads

28328456

Reads aligned (%)

93.3

Duplicates removed (%)

11.1

Number of peaks

19918 (qval < 1E-05)