Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

MEF_p20

cell type

MEF derived p20 iPS

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP experiments were performed as described previously (van Oevelen et al., 2008) using an antibody against H3K27ac (ab4729, Abcam) and CTCF (Millipore, 07-729). DNA libraries were prepared using Illumina's reagents and instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

35227719

Reads aligned (%)

93.3

Duplicates removed (%)

16.4

Number of peaks

28052 (qval < 1E-05)

mm9

Number of total reads

35227719

Reads aligned (%)

93.1

Duplicates removed (%)

16.5

Number of peaks

28085 (qval < 1E-05)