Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
MEF_p20_2
cell type
MEF derived p20 iPS

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed as described previously (van Oevelen et al., 2008) using an antibody against H3K27ac (ab4729, Abcam) and CTCF (Millipore, 07-729). DNA libraries were prepared using Illumina's reagents and instructions.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
36864795
Reads aligned (%)
46.1
Duplicates removed (%)
25.8
Number of peaks
48432 (qval < 1E-05)

mm9

Number of total reads
36864795
Reads aligned (%)
46.0
Duplicates removed (%)
26.0
Number of peaks
48383 (qval < 1E-05)

Base call quality data from DBCLS SRA