Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
Macrophage-derived iPS cells
NA
NA

Attributes by original data submitter

Sample

source_name
macrophage_p20_2
cell type
macrophage derived p20 iPS

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments were performed as described previously (van Oevelen et al., 2008) using an antibody against H3K27ac (ab4729, Abcam) and CTCF (Millipore, 07-729). DNA libraries were prepared using Illumina's reagents and instructions.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
22653790
Reads aligned (%)
61.1
Duplicates removed (%)
11.9
Number of peaks
46623 (qval < 1E-05)

mm9

Number of total reads
22653790
Reads aligned (%)
60.9
Duplicates removed (%)
12.0
Number of peaks
46617 (qval < 1E-05)

Base call quality data from DBCLS SRA