Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Bone

Cell type

Chondrocytes

MeSH Description

Polymorphic cells that form cartilage.

Sample

source_name

Mouse primary chodrnocytes

tisse

rib cartilage

cell type

chondrocytes

chip antibody

H3K27me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell preparation was performed according to the SimpleCHIP enzymatic chromatin IP kit (# 9003 Cell Signaling Technology). ChIP was performed using the H3K27me3 antibody (# 9733n Cell Signaling Technology) ChIP'ed DNA and Input DNA were sequenced by Otogenetics (Necros, GA)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

20000010

Reads aligned (%)

97.7

Duplicates removed (%)

7.5

Number of peaks

117 (qval < 1E-05)

mm9

Number of total reads

20000010

Reads aligned (%)

97.7

Duplicates removed (%)

7.5

Number of peaks

96 (qval < 1E-05)