Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CEBPB

Cell type

Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
A549
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
A549
cell organism
human
cell description
epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
CEBPB
antibody antibodydescription
Rabbit polyclonal IgG. Antibody Target: CEBPB
antibody targetdescription
epitope mapping at the C-terminus of C/EBP-beta of rat origin
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-150
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH002819
replicate
1

Sequenced DNA Library

library_name
GSM935630: Stanford_ChipSeq_A549_CEBPB_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
54249193
Reads aligned (%)
90.3
Duplicates removed (%)
7.7
Number of peaks
61407 (qval < 1E-05)

hg38

Number of total reads
54249193
Reads aligned (%)
92.1
Duplicates removed (%)
6.5
Number of peaks
61856 (qval < 1E-05)

Base call quality data from DBCLS SRA