Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SREBF1

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HepG2
cell organism
human
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
insulin
treatment description
DMEM with 0.5% BSA supplemented with 100 nM insulin and 10 uM 22-hydroxycholesterol for 6 h. (Snyder)
antibody
SREBP1
antibody antibodydescription
Rabbit polyclonal. Antibody Target: SREBP1
antibody targetdescription
Sterol regulatory element binding transcription factor 1
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-8984
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000756
labversion
remapped from hg18, Illumina 2.0, low confidence sequences excluded. Data paired with HepG2_Insulin_Control.
replicate
1

Sequenced DNA Library

library_name
GSM935627: Stanford_ChipSeq_HepG2_insulin_SREBP1_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
11958882
Reads aligned (%)
89.0
Duplicates removed (%)
3.7
Number of peaks
4427 (qval < 1E-05)

hg38

Number of total reads
11958882
Reads aligned (%)
89.5
Duplicates removed (%)
3.5
Number of peaks
4406 (qval < 1E-05)

Base call quality data from DBCLS SRA