Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BHLHE40

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
BHLHE40_(NB100-1800)
antibody antibodydescription
Rabbit polyclonal, maps to a region between residues 350 and the C-terminus (residue 412). Antibody Target: BHLHE40
antibody targetdescription
This gene encodes a basic helix-loop-helix protein expressed in various tissues. Expression in the chondrocytes is responsive to the addition of Bt2cAMP. The encoded protein is believed to be involved in the control of cell differentiation.
antibody vendorname
Novus
antibody vendorid
NB100-1800
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
replicate
1

Sequenced DNA Library

library_name
GSM935616: Stanford_ChipSeq_K562_BHLHE40_(NB100-1800)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
79193277
Reads aligned (%)
89.6
Duplicates removed (%)
6.0
Number of peaks
35332 (qval < 1E-05)

hg19

Number of total reads
79193277
Reads aligned (%)
89.0
Duplicates removed (%)
7.2
Number of peaks
35109 (qval < 1E-05)

Base call quality data from DBCLS SRA