Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAFK

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HepG2
cell organism
human
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
MafK_(SC-477)
antibody antibodydescription
Rabbit polyclonal raised against epitope mapping at the C-terminus of NF-E2 p18 of mouse origin; recommended for detection of NF-E2 p18 (MafK) of mouse, rat and human origin. Antibody Target: MafK
antibody targetdescription
NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG, or MafK) and p45. Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP)proteins. Since they lack a putative transactivation domain, small Mafs behave as transcriptional repressors when they dimerize among themselves. They serve as transcriptional activators by dimerizing with other (usually larger) bZip proteins and recruiting them to specific DNA-binding sites.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
SC-477
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001791
replicate
1

Sequenced DNA Library

library_name
GSM935610: Stanford_ChipSeq_HepG2_MafK_(SC-477)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
65745330
Reads aligned (%)
84.6
Duplicates removed (%)
5.8
Number of peaks
55426 (qval < 1E-05)

hg38

Number of total reads
65745330
Reads aligned (%)
86.5
Duplicates removed (%)
4.5
Number of peaks
56584 (qval < 1E-05)

Base call quality data from DBCLS SRA