Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRF1

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa-S3
biomaterial_provider
ATCC
lab
Harvard
lab description
Struhl - Harvard University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HeLa-S3
cell organism
human
cell description
cervical carcinoma
cell karyotype
cancer
cell lineage
ectoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
BRF1
antibody antibodydescription
Rabbit polyclonal - Anti-BRF antibody 128 was raised by immunising rabbits with synthetic peptide KISSKINYSVLRGLS (human BRF residues 533-547) coupled to keyhole limpet haemocyanin. Antibody Target: BRF1
antibody targetdescription
'B-related factor 1', subunit of RNA polymerase III transcription initiation factor IIIB
antibody vendorname
Donated by Dr. RJ White, The Beatson Institute for Cancer Research
antibody vendorid
128
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000612
replicate
1

Sequenced DNA Library

library_name
GSM935582: Harvard_ChipSeq_HeLa-S3_BRF1_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
59396606
Reads aligned (%)
72.5
Duplicates removed (%)
9.5
Number of peaks
1073 (qval < 1E-05)

hg19

Number of total reads
59396606
Reads aligned (%)
71.7
Duplicates removed (%)
11.1
Number of peaks
1207 (qval < 1E-05)

Base call quality data from DBCLS SRA