Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293
biomaterial_provider
ATCC
lab
Yale
lab description
Snyder - Yale University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HEK293
cell organism
human
cell description
embryonic kidney, cells contain Adenovirus 5 DNA (PMID: 11967234)
cell karyotype
normal
cell lineage
mesoderm
cell sex
U
treatment
None
treatment description
No special treatment or protocol applies
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
control
std
control description
Standard input signal for most experiments.
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEH000631
replicate
1

Sequenced DNA Library

library_name
GSM935534: Yale_ChipSeq_HEK293_Pol2_std
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
11033874
Reads aligned (%)
82.7
Duplicates removed (%)
19.9
Number of peaks
15423 (qval < 1E-05)

hg19

Number of total reads
11033874
Reads aligned (%)
82.1
Duplicates removed (%)
20.1
Number of peaks
15490 (qval < 1E-05)

Base call quality data from DBCLS SRA