Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
K562
cell organism
human
cell description
leukemia, "The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises." - ATCC
cell karyotype
cancer
cell lineage
mesoderm
cell sex
F
treatment
None
treatment description
No special treatment or protocol applies
antibody
Pol2(phosphoS2)
antibody antibodydescription
Rabbit polyclonal against peptide conjugated to KLH derived from within residues 1600 - 1700 of S. cerevisiae RNA polymerase II CTD repeat YSPTSPS, phosphorylated at S2. Antibody Target: POL2S2
antibody targetdescription
RNA polymerase II, large subunit- specific for phosphorylated C-terminal domain.
antibody vendorname
Abcam
antibody vendorid
ab5095
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001795
replicate
1

Sequenced DNA Library

library_name
GSM935402: Stanford_ChipSeq_K562_Pol2(phosphoS2)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
55430178
Reads aligned (%)
99.2
Duplicates removed (%)
4.8
Number of peaks
13270 (qval < 1E-05)

hg19

Number of total reads
55430178
Reads aligned (%)
98.9
Duplicates removed (%)
5.2
Number of peaks
13181 (qval < 1E-05)

Base call quality data from DBCLS SRA