Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MAFF

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
HepG2
cell organism
human
cell description
hepatocellular carcinoma
cell karyotype
cancer
cell lineage
endoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
MafF_(M8194)
antibody antibodydescription
Rabbit polyclonal. Raised against synthetic peptide corresponding to amino acids 147-163 of human MAFF. The immunizing peptide does not cross-react with other members of the family. Antibody Target: MafF
antibody targetdescription
The protein encoded by this gene is a (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. Can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. May also be involved in the cellular stress response.
antibody vendorname
Sigma-Aldrich
antibody vendorid
M8194
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001791
replicate
1

Sequenced DNA Library

library_name
GSM935306: Stanford_ChipSeq_HepG2_MafF_(M8194)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
63610657
Reads aligned (%)
65.6
Duplicates removed (%)
7.6
Number of peaks
61670 (qval < 1E-05)

hg38

Number of total reads
63610657
Reads aligned (%)
67.1
Duplicates removed (%)
6.4
Number of peaks
62651 (qval < 1E-05)

Base call quality data from DBCLS SRA