Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CHD1

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H1-hESC
cell organism
human
cell description
embryonic stem cells
cell karyotype
normal
cell lineage
inner cell mass
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
CHD1_(A301-218A)
antibody antibodydescription
Rabbit Polyclonal Antigen Affinity Purified, Unconjugated, Liquid. Antibody Target: CHD1
antibody targetdescription
CHD1 is a member of the CHD (chromodomain-helicase-DNA-binding) family of proteins that interacts with nucleosomes and plays a role in chromatin remodeling to modulate transcription. The members of the CHD family of proteins possess 3 common structural and functional domains: a chromodomain (chromatin organization modifier), an SNF2-like helicase/ATPase domain, and a C-terminal DNA-binding domain. CHD1 has been shown to interact with the transcriptional corepressor NCoR and histone deacetylase 1 indicating a role in transcriptional regulation. CHD1 has also been shown to interact with the Paf1 complex and Rtf1 implicating an additional role in transcriptional elongation. Alternate names for CHD1 include chromodomain-helicase-DNA-binding protein 1, ATP-dependent helicase CHD1, and DKFZp686E2337.
antibody vendorname
Bethyl Laboratories
antibody vendorid
A301-218A
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001834
replicate
1

Sequenced DNA Library

library_name
GSM935296: Stanford_ChipSeq_H1-hESC_CHD1_(A301-218A)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
56301050
Reads aligned (%)
89.5
Duplicates removed (%)
7.7
Number of peaks
1972 (qval < 1E-05)

hg38

Number of total reads
56301050
Reads aligned (%)
91.9
Duplicates removed (%)
6.0
Number of peaks
1948 (qval < 1E-05)

Base call quality data from DBCLS SRA