Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ATF4

Cell type

Cell type Class
Neural
Cell type
GBM
NA
NA

Attributes by original data submitter

Sample

source_name
GBM cell line
cell type
GBM cell lines
chip antibody
ATF4(Cell Signaling Technology,#11815)
treatment
glucose 25mM

Sequenced DNA Library

library_name
GSM6062644
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) assay was performed by using the SimpleChIP Enzymatic Chromatin IP kit (#9003, Cell Signaling Technology) according to the manufacturer's instructions. Sequencing libraries were prepared using the NEBNext DNA Library Prep Master Mix Set (Illumina) according to the manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
20687435
Reads aligned (%)
92.0
Duplicates removed (%)
15.5
Number of peaks
308 (qval < 1E-05)

hg19

Number of total reads
20687435
Reads aligned (%)
91.7
Duplicates removed (%)
15.6
Number of peaks
208 (qval < 1E-05)

Base call quality data from DBCLS SRA