Antigen

Antigen Class

TFs and others

Antigen

TRIM28

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7 cells

cell line

MCF7

treatment

Control

antibody

TRIM28

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, MCF7 lysates with were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. For RNA-seq, RNA was harvested using Trizol reagent from control, EZH2, and TRIM28 depleted MCF7 cells. RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

44442792

Reads aligned (%)

95.2

Duplicates removed (%)

5.7

Number of peaks

1882 (qval < 1E-05)

hg19

Number of total reads

44442792

Reads aligned (%)

93.5

Duplicates removed (%)

8.3

Number of peaks

1407 (qval < 1E-05)