submitter states "custom" (no meaningful entry) DNeasy Blood and Tissue Kit DNA extraction Parallel PCR reactions to assay 151 imprinted differentially methylated domains (Supplementary material in manuscript) with a final concentration of 1xHotStar PCR buffer, 0.2mM dNTPs, 1U HotStar taq and 0.4uM each primer pair and 2ng DNA were performed in a 15uL reaction volume in 384-well plates. The plates were prepared on the Agilent Bravo Workstation. The cycling conditions included a 15 minute 95°C denaturation, followed by 30 cycles of 95°C for 30 seconds, 50°C for 30 seconds, 72°C for 90 seconds, with a 5 minute final extension at 72°C and were performed on the C1000 Touch Thermal Cycler. PCR products were pooled for each sample and SPRI-purified using Sera-Mag carboxylate-modified Magnetic SpeedBeads as per manufacturer’s recommendations. A-overhang addition was done using 1x NEB buffer 2, 0.2mM dATP, 12.5U Klenow fragment (3’->5’ exo-) in a 25uL reaction incubated at 37°C for 30 minutes. Following SPRI purification, Illumina adapters were ligated using the Quick ligation kit in a 40uL reaction. Following another SPRI purification, libraries were amplified (11 cycles) and barcoded using the Sanger 8-base index in a 50uL reaction containing: 1x High Fidelity buffer, 1U Phusion High Fidelity taq, 0.2mM dNTPs, 0.1uM each primer . Final libraries were SPRI purified and concentration and quality were assessed by the 2100 Bioanalyzer system and Kapa library quantification. The 48 barcoded libraries were pooled and multiplexed.