Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD4

Cell type

Cell type Class
Others
Cell type
NC797
NA
NA

Attributes by original data submitter

Sample

source_name
797 BRD4-NUT
cell line
797 BRD4-NUT
genotype
N-BioTAP-cMYC
treatment
JQ1

Sequenced DNA Library

library_name
GSM6049330
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were formaldehyde fixed in 3.7% formaldehyde. DNA-protein complexes were affinity purified and DNA was extracted as described for the BioTAP-XL procedure in Alekseyenko et al 2015. NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina (New England Biolabs cat#E6240S)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
19983059
Reads aligned (%)
97.8
Duplicates removed (%)
1.8
Number of peaks
826 (qval < 1E-05)

hg19

Number of total reads
19983059
Reads aligned (%)
97.1
Duplicates removed (%)
2.9
Number of peaks
931 (qval < 1E-05)

Base call quality data from DBCLS SRA