Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC H9

NA

NA

Sample

source_name

human embryonic stem cells

cell type

hESC

passages

18-21

strain

H9

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin were sheared to fragements by using different strategy for histone modifications(MNase-digested) and Sox2(Sonication),then histone-DNA or Sox2-DNA complexes were isolated with corresponding antibody. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

74783511

Reads aligned (%)

96.7

Duplicates removed (%)

3.2

Number of peaks

2086 (qval < 1E-05)

hg19

Number of total reads

74783511

Reads aligned (%)

95.8

Duplicates removed (%)

5.3

Number of peaks

1733 (qval < 1E-05)