GSM6046557: chpseq-FA71xAsnSer-1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SIRT6
Cell type
Cell type Class
Unclassified
Cell type
Unclassified
NA
NA
Attributes by original data submitter
Sample
source_name
patient FA71x chipseq technical replicate 1
disease status
Friedreich Ataxia
genotype
AsnSer
antibody
SIRT12
Sequenced DNA Library
library_name
GSM6046557
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
CHIPseq libraries were prepared for sequencing using standard Illumina protocols RNA was isolated using the RNeasy mini kit (QIAgen, Hilden, Gemany). For ChIPseq cells were crosslinked with 1% formaldehyde directly added to tissue culture media and lysed in 5mM PIPES pH 8.0, 85 mM KCL and 0.5% NP40 and cell nuclei recovered. Nuclei were lysed in 1% SDS, 10 mM EDTA pH 8.0 and 50 mM Tris-HCL, pH 8.0. Nuclei were sonicated to a DNA average size of 300bp. IP was performed with Sirt6 antibody from Abcam (Cambridge UK) ab191385