Antigen

Antigen Class

TFs and others

Antigen

SIRT6

Cell type

Cell type Class

Unclassified

Cell type

Unclassified

NA

NA

Sample

source_name

patient 3816x chipseq technical replicate 1

disease status

Friedreich Ataxia

genotype

Asn

antibody

SIRT6

Sequenced DNA Library

library_name

GSM6046551

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

CHIPseq libraries were prepared for sequencing using standard Illumina protocols RNA was isolated using the RNeasy mini kit (QIAgen, Hilden, Gemany). For ChIPseq cells were crosslinked with 1% formaldehyde directly added to tissue culture media and lysed in 5mM PIPES pH 8.0, 85 mM KCL and 0.5% NP40 and cell nuclei recovered. Nuclei were lysed in 1% SDS, 10 mM EDTA pH 8.0 and 50 mM Tris-HCL, pH 8.0. Nuclei were sonicated to a DNA average size of 300bp. IP was performed with Sirt6 antibody from Abcam (Cambridge UK) ab191385

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

8905205

Reads aligned (%)

80.8

Duplicates removed (%)

17.5

Number of peaks

379 (qval < 1E-05)

hg19

Number of total reads

8905205

Reads aligned (%)

80.1

Duplicates removed (%)

18.3

Number of peaks

359 (qval < 1E-05)