Curated Sample Data


Genome
mm9
Antigen Class
Input control
Antigen
Input control
Cell type Class
Blood
Cell type
Hematopoietic Stem Cells

Cell type information


MeSH Description
Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Attributes by Original Data Submitter


source_name
HSPCs
cell type
hematopoietic stem and progenitor cells
genotype/variation
WT
strain
129Sv/B6
chip antibody 1
none (input)
chip antibody 2
none (input)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells from bone marrow were isolated from mice and eriched for c-Kit+ cells,crosslinked for 10 minutes with 1% formaldehyde and stained with Lineage antibodies (CD11b, Gr1, Ter119, B220, CD4, CD8), Sca1 and c-Kit and then sorted and snap frozen Libraries were generated as previously published (Lara Astiaso et al, 2014) Briefly, sonicated chromatin was immunoprecipitated with H3 and libraries were generated on chromatin bound to beads. Libaries with multiplexed with NextFlex Barcoded primers from Bioo Scientific. ChIp with specific histone modifications were performed with barcoded chromatin and released DNA was amplified with ilumina standard primers

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
23793642
Reads aligned (%)
98.2
Duplicates removed (%)
13.3
Number of peaks
464 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA