Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TP53

Cell type

Cell type Class
Breast
Cell type
Mammary epithelial cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mammary Epithelial Cells
cell type
Mammary Epithelial Cells
genotype
wild type
strain
Normal Human Mammary Epithelial Cells CC-2551
treatment condition
activated p53
chip antibody
Cell Signaling technologies, p53(1C12) mAb#2524

Sequenced DNA Library

library_name
GSM6038092
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was purified by Phenol:Chloroform extraction. Immunoprecipitated DNA was subjected to library preparation (NEBNext Ultra II DNA Library Prep Kit, Illumina). In the library preparation protocol, samples were amplified using 12 PCR cycles.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
43199472
Reads aligned (%)
96.0
Duplicates removed (%)
51.3
Number of peaks
1014 (qval < 1E-05)

hg19

Number of total reads
43199472
Reads aligned (%)
95.2
Duplicates removed (%)
52.7
Number of peaks
1095 (qval < 1E-05)

Base call quality data from DBCLS SRA