GSM6022003: PC3 HIF2A ChIP rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EPAS1
Cell type
Cell type Class
Prostate
Cell type
PC-3
Primary Tissue
Prostate
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
PC3 Prostate cancer cell line
cell line
PC3
ambient o2 concentration
0.50%
duration of hypoxia
16 hours
chip antibody
HIF-2a (rabbit polyclonal, PM9)
Sequenced DNA Library
library_name
GSM6022003
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) experiments were performed using antibodies directed against HIF-1a (rabbit polyclonal, PM14), HIF-2a (rabbit polyclonal, PM9), or HIF-1b (rabbit polyclonal, Novus Biologicals, NB100-110). Automated library preparation was performed using the Apollo prep system (Takara, PrepX Comp ILMN 32i DNA Lib 96).