Antigen

Antigen Class

TFs and others

Antigen

EPAS1

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

HCT116 Colorectal cancer cell line

cell line

HCT116

ambient o2 concentration

0.50%

duration of hypoxia

16 hours

chip antibody

HIF-2a (rabbit polyclonal, PM9)

Sequenced DNA Library

library_name

GSM6021999

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation (ChIP) experiments were performed using antibodies directed against HIF-1a (rabbit polyclonal, PM14), HIF-2a (rabbit polyclonal, PM9), or HIF-1b (rabbit polyclonal, Novus Biologicals, NB100-110). Automated library preparation was performed using the Apollo prep system (Takara, PrepX Comp ILMN 32i DNA Lib 96).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

27383936

Reads aligned (%)

96.0

Duplicates removed (%)

11.0

Number of peaks

903 (qval < 1E-05)

hg19

Number of total reads

27383936

Reads aligned (%)

95.1

Duplicates removed (%)

11.2

Number of peaks

651 (qval < 1E-05)