Antigen

Antigen Class

TFs and others

Antigen

ESRRA

Cell type

Cell type Class

Breast

Cell type

BT-474

Primary Tissue

Breast

Site of Extraction

Soft Tissue

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

BT474 human breast cancer cell line treated with vehicle for 90 minutes

cell line

BT474

tissue type

Breast tumor

chip antibody

ERRa

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

After 90min treatment with vehicle or AICAR, nuclei were isolated and subjected to chromatin immunoprecipitation. Following ERRa ChIP, genomic DNA was purified with QIAGEN DNA extraction kit. Libraries were performed at the Génome Québec Innovation Centre (McGill University) using standard protocol for HiSeq - Illumina Single reads 50bp sequencing lane

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

105183446

Reads aligned (%)

90.5

Duplicates removed (%)

27.2

Number of peaks

9512 (qval < 1E-05)

hg19

Number of total reads

105183446

Reads aligned (%)

89.6

Duplicates removed (%)

29.0

Number of peaks

9302 (qval < 1E-05)