GSM6016067: MCF7 shEV+E2 ER ChIP-seq rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
ESR1
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
breast cancer MCF7 cells
cell line
MCF7
cell type
breast cancer MCF7 cells
treatment
treated with E2
RNAi
EV
chip antibody
anti-ER
Sequenced DNA Library
library_name
GSM6016067
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-seq: Total RNA was extracted from cell pellets using RNAzol reagent. For ChIP-seq: 1% formaldehyde in PBS was used to crosslink the cells. Antibody enrich DNA fragments overnight from Sonicated lysates. RNA and ChIP libraries were prepared for sequencing using standard Illumina protocols