Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Bone

Cell type

Chondrocytes

MeSH Description

Polymorphic cells that form cartilage.

Sample

source_name

K27ac CHiP for hdac3-knockout Cre sample

strain

C57BL/6

cell type

Chondrocytes

genotype/variation

hdac3-knockout

transduction

Cre-recombinase adenovirus

chip antibody

Anti-H3K27Ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody Libraries were prepared according to NuGene Ultralow library prep kit V2

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

14518929

Reads aligned (%)

99.0

Duplicates removed (%)

2.5

Number of peaks

73 (qval < 1E-05)

mm9

Number of total reads

14518929

Reads aligned (%)

98.9

Duplicates removed (%)

2.6

Number of peaks

53 (qval < 1E-05)