Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
H1 TKO embryonic stem cells, H3K4me3 ChIP
strain/background
C57BL/6
cell type
embryonic stem cell line
genotype/variation
H1c, H1d, H1e triple-null
chip antibody
H3K4me3 (Abcam ab8580, lot# GR111321-2)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
TruSeq DNA sample preparation kit (Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
20223225
Reads aligned (%)
39.7
Duplicates removed (%)
8.6
Number of peaks
16189 (qval < 1E-05)

mm9

Number of total reads
20223225
Reads aligned (%)
39.6
Duplicates removed (%)
8.7
Number of peaks
16080 (qval < 1E-05)

Base call quality data from DBCLS SRA