Antigen

Antigen Class

TFs and others

Antigen

Dmc1

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Testis

maternal strain

PWD/PhJ

paternal strain

B10.F-H2pb1/(13R)J

antibody

Anti-DMC1 Santa Cruz (C-20, sc 8973)

sequencing technique

SSDS

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For DMC1 SSDS, testicular samples were obtained frozen and were directly thawed in 1% paraformaldehyde and gently dissociated. Genomic DNA for whole genome sequencing was extracted from the testicular samples before fixation with the DNeasy Blood and Tissue kit (Qiagen). DMC1 ChIP was performed as described previously (Smagulova et al., 2011; Khil et al., 2012; Brick et al., 2012) with minor modifications. Sequencing libraries for anti-DMC1 were prepared following the method described in (Khil et al., 2012). Single Stranded DNA Sequencing (SSDS)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

205355312

Reads aligned (%)

32.1

Duplicates removed (%)

47.0

Number of peaks

6885 (qval < 1E-05)

mm9

Number of total reads

205355312

Reads aligned (%)

32.1

Duplicates removed (%)

47.1

Number of peaks

6850 (qval < 1E-05)