Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

MEL

Tissue

Blood

Lineage

cellLine

Description

Leukemia (K562 analog)

Sample

source_name

MEL

biomaterial_provider

Weissman lab

lab

PSU-m

lab description

Hardison - Penn State University

datatype

ChipSeq

datatype description

Chromatin IP Sequencing

cell

MEL

cell organism

mouse

cell description

Leukemia (K562 analog)

cell sex

U

antibody

Pol2-4H8

antibody antibodydescription

Mouse monoclonal to RNA polymerase II CTD repeat YSPTSPS antibody (4H8) - ChIP Grade. Antibody Target: POL24H8

antibody targetdescription

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes

antibody vendorname

Abcam

antibody vendorid

ab5408

age

immortalized

age description

Immortal cells

control

Input

control description

Control signal which may be subtracted from experimental raw signal before peaks are called.

controlid

wgEncodeEM001929

replicate

1

strain

C57BL/6

strain description

C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name

GSM923577: PSU_ChipSeq_MEL_Pol2-4H8

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

54812883

Reads aligned (%)

95.8

Duplicates removed (%)

12.7

Number of peaks

7321 (qval < 1E-05)

mm9

Number of total reads

54812883

Reads aligned (%)

95.7

Duplicates removed (%)

12.8

Number of peaks

7265 (qval < 1E-05)