Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Cp190

Cell type

Cell type Class
Cell line
Cell type
Cultured cell lines
NA
NA

Attributes by original data submitter

Sample

source_name
Cultured cells
cell line
Ras3
mutation
none
antibody
anti-Cp190 from Whitfield et al. 1988

Sequenced DNA Library

library_name
GSM5956445
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Live cells were crosslinked with 1.8% formaldehyde for 10 min at 25C. Soluble chromatin was prepared and immunoprecipitation performed as described in Kahn et al. Nucleic Acids Res. 2016. 44:10132-10149 2ng of immunoprecipitated DNA were processed using NEBNext Ultra II DNA Library preparation Kit for Illumina (cat# E76455) and index oligonucleotides from NEBNext Multiplex Oligos for Illumina (cat# E7335).

Sequencing Platform

instrument_model
HiSeq X Ten

dm6

Number of total reads
28690631
Reads aligned (%)
94.5
Duplicates removed (%)
44.6
Number of peaks
13745 (qval < 1E-05)

dm3

Number of total reads
28690631
Reads aligned (%)
94.7
Duplicates removed (%)
43.4
Number of peaks
13727 (qval < 1E-05)

Base call quality data from DBCLS SRA