Antigen

Antigen Class

TFs and others

Antigen

mod(mdg4)

Cell type

Cell type Class

Cell line

Cell type

Cultured cell lines

NA

NA

Sample

source_name

Cultured cells

cell line

CP-R6

mutation

Cp190[3]

antibody

anti-Mod(Mdg4) from Gerasimova et al. 1998

Sequenced DNA Library

library_name

GSM5956422

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Live cells were crosslinked with 1.8% formaldehyde for 10 min at 25C. Soluble chromatin was prepared and immunoprecipitation performed as described in Kahn et al. Nucleic Acids Res. 2016. 44:10132-10149 2ng of immunoprecipitated DNA were processed using NEBNext Ultra II DNA Library preparation Kit for Illumina (cat# E76455) and index oligonucleotides from NEBNext Multiplex Oligos for Illumina (cat# E7335).

Sequencing Platform

instrument_model

HiSeq X Ten

dm6

Number of total reads

14545669

Reads aligned (%)

88.1

Duplicates removed (%)

38.5

Number of peaks

20906 (qval < 1E-05)

dm3

Number of total reads

14545669

Reads aligned (%)

89.3

Duplicates removed (%)

36.7

Number of peaks

21237 (qval < 1E-05)