Antigen

Antigen Class

Histone

Antigen

H3.3

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Cg_Anti-H3.3_ChIP-seq

strain

B6.SPRET7MOLF12

genotype/variation

control

mouse age

6 weeks

tissue

testis

chip antibody

Millipore:ChIPAb+TM histone H3.3-ChIP validated antibody (cat 17-10245)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted from whole testis after sonication and precipitation with antibody Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit by the service platform of McGill University and genome Quebec Innovation Centre

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

42133955

Reads aligned (%)

97.9

Duplicates removed (%)

10.1

Number of peaks

634 (qval < 1E-05)

mm9

Number of total reads

42133955

Reads aligned (%)

97.7

Duplicates removed (%)

10.2

Number of peaks

655 (qval < 1E-05)