Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
ES-Bruce4
biomaterial_provider
University of California, San Diego Transgenic Core and Embryonic Stem Cell
lab
LICR-m
lab description
Ren - Ludwig Institue for Cancer Research
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
ES-Bruce4
cell organism
mouse
cell description
mouse embryonic stem cells
cell sex
M
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
age
E0
age description
Embryonic day 0 (stem cell)
age
E0
age description
Embryonic day 0 (stem cell)
control
std
control description
Standard input signal for most experiments.
controlid
wgEncodeEM001683
labversion
12/23/09 SOLEXA1 Lane 1
replicate
1
softwareversion
Bowtie
strain
C57BL/6
strain description
C57 black 6, the most common inbred strain of laboratory mouse

Sequenced DNA Library

library_name
GSM918749: LICR_ChipSeq_ES-Bruce4_Pol2_E0
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
38482583
Reads aligned (%)
69.3
Duplicates removed (%)
28.6
Number of peaks
15283 (qval < 1E-05)

mm9

Number of total reads
38482583
Reads aligned (%)
68.8
Duplicates removed (%)
28.4
Number of peaks
15740 (qval < 1E-05)

Base call quality data from DBCLS SRA