GSM5929910: Pre LNCaP FOXA1 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOXA1
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
prostate cancer cell line
cell line
LNCaP
chip antibody
FOXA1 (Abcam, ab5089)
replicate
2
treatment
Untreated
Sequenced DNA Library
library_name
GSM5929910
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitations were performed as described previously (Stelloo et al., 2018). Samples were crosslinked in 1% formaldehyde (Merck) for 10 minutes and subsequently quenched with 2.5 M glycine. Samples were lysed as described (Schmidt et al., 2009) and sonicated for at least 10 cycles of 30 sec on, 30 sec off using a Diagenode Bioruptor Pico. For each ChIP, 5 µg of antibody was conjugated with 50 µL magnetic protein A or G beads (10008D or 10009D, Thermo Fisher Scientific). Antibodies used were FOXA1 (ab5089, Abcam) and ARNTL (ab93806, Abcam). Immunoprecipitated DNA was processed for library preparation using a KAPA library preparation kit (KK8234, Roche)