Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562 PBS Vehicle Control DNase-seq

cell line

K562

cell type

myelogenous leukemia cell line

treated with

0.5% 1xPBS vehicle

duration of treatment

72 hours

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

30-50 million nuclei were digested with a range of recombinant DNase I enzyme (Roche) concentrations (between 1.2 and 12U) for 15 minutes at 37°C in 120uL 1X DNase buffer. Libraries constructed according to PMID: 20150154 Cell pellets were washed twice with 1xPBS and nuclei isolated by 0.1% NP-40 lysis

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

196641847

Reads aligned (%)

0.2

Duplicates removed (%)

8.5

Number of peaks

59 (qval < 1E-05)

hg19

Number of total reads

196641847

Reads aligned (%)

0.2

Duplicates removed (%)

9.9

Number of peaks

73 (qval < 1E-05)