Antigen

Antigen Class

TFs and others

Antigen

Jun

Cell type

Cell type Class

Digestive tract

Cell type

Intestines

MeSH Description

The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.

Sample

source_name

Whole Intestine

antibody

cJUN Polyclonal Rabbit antibody (Santa Cruz sc-44)

strain

C57BL/6

age

E14.5

genotype

wild type

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed following a previously described protocol (Vokes et. Al, 2007). Intestines wer snap-frozen in liquid and ground in a mortar and pestle to dissociate the tissue to single cells. DNA and protein were then crosslinked and sonicated to an average 500 bp fragment size. cJUN bound regions were immunoprecipitated overnight using anti cJUN Polyclonal Rabbit antibody (Santa Cruz sc-44) bound to Protein G Dyanabeads). Input control consisted of sonicated DNA. ChIP libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

40532069

Reads aligned (%)

89.4

Duplicates removed (%)

72.0

Number of peaks

443 (qval < 1E-05)

mm9

Number of total reads

40532069

Reads aligned (%)

89.2

Duplicates removed (%)

72.1

Number of peaks

450 (qval < 1E-05)